The performance of the thermostable enzyme mixtures was studied in hydro­lysis experiment in test tubes (5 mL). Enzyme mixtures were dosed on the basis of FPU activity to the substrates (10 gL-1 dry matter) suspended in 50 mM sodium acetate, pH 5. The standard enzyme dosage was 10 FPUg-1 cellulose. Triplicate samples were incubated with mixing at 35 °C, 45 °C, 55 °C or 60 °C for 24 h, 48 h or 72 h. Reference samples with inactivated enzymes and corresponding substrates were also prepared.

Chemical Analysis

The release of hydrolysis products was measured as reducing sugars assayed by the DNS method using glucose as standard [10]. The results were cor­rected by taking into account the blank samples containing corresponding amounts of inactivated enzymes and substrate. The mono — and oligosac­charides formed were also analysed by high-performance anion-exchange chromatography on a Dionex 4500i series chromatograph with pulsed amper — ometric detection (HPAEC-PAD), as described earlier [75].

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