Hydrolytic Properties of T. reesei Enzymes at High Temperatures

The hydrolysis experiments were carried out at a substrate consistency of 10 gL-1 in 50 mM sodium acetate, pH 5, in a volume of 100 mL, and incubated in shake flasks with shaking (200 rpm) at different temperatures from 55 °C to 70 °C. Duplicate shake flasks were sampled (5 mL sample) at 2 h, 4 h, 6 h, 24 h, 48 h and 72 h from the start of the hydrolysis. Possible evaporation was checked by weighing and corrected when necessary by adding a correspond­ing amount of water. The release of hydrolysis products was followed during the hydrolysis.