One of the key factors in the success of microbial-mediated processes is an adequate understanding of process microbial, more specifically the study of microscopic organisms involved in wastewater degradation and byproduct formation. The low growth rate, the specific nutrient and trace mineral requirements of methanogens, coupled with their susceptibility to changes in environmental conditions demand meticulous process control for stable operation (Khanal, 2008). The biochemistry mainly involves enzyme-mediated chemical changes (the chemical activities of microorganism), type of substrate (kind wastewater) microorganism can destroy or transform to new compounds, and the step-by­step pathway of degradation (Sachdeva et al., 2000).