• Materials Sugarcane stems 5kg Dry yeast, 15g

• Items

Brix meter, 5L flask, Dimroth condenser, Liebig condenser, Stick, Beaker Cloth filter

1. Fermentation method

2. Mill juice out of Sugarcane stems. (about 3L of juice)

3. The juice is filtered out impurities.

4. Measurement Brix of juice.

5. Dry yeast is added to juice, the rate of 6g/L.

6. It keeps in the flask which sealed except the vent.

7. A cover is opened one day and once, then juice and dry yeast mixes so that air may en­ter with stick.

8. It continues until Brix becomes fixed.

9. Distillation method (Fig. 8)

10. Fermented juice is filtered out sediment.

11. It heats to boiling point in distiller.

12. Dimroth condenser is kept warm (about 70 degree) with hot water which is made to cir­culate by a pump.

13. Allihn condenser cools with tap water (about 20 degree).

14. Bio-ethanol which falls from the point of a allihn condenser is caught with beaker on ice.

1.4.1. Qualitative analysis for ethanol

Iodoform test on cold is special test for ethanol as the following: I ml ethanol layer mix with iodide and sodium hydroxide after that, the presence of yellow crystal and iodoform odor produced, this meaning presence of ethanol.

Saccharinitv Material sugarcane, sugar beet, etc.
Ethanol alcoholicitv after fermentation
compression
Over 99. 5%
Fermen -tation	abso ute
saccharifi’ cation
grinding
distillation	ethanol
enzyme
yeast
Cellulosic material
grinding
saccharification
wood remains, lumber residues, thinned wood, etc
hydrolyzation by
sulfuric acid
Figure 6. Production of absolute ethanol from Saccharinity, Starch and Cellulosic materials

thprmnmPtFr
C Did nutir eat
litblg cqnQfП1ЭГ
fraeticnatiii|E "column
eald water in
dii till feta
іпй — Ьишйійі tf irttUJii
HEAT
Figure 8. The distillation process for ethanol production.
3.2.4. Quantitative ethanol determination З.2.4.1. Direct injected GC method Beverage sample solution (0.5 mL) was dispensed into an l-mL caped sample vial, and then 5 mL of 1% internal standard solution (equivalent to 50 mg) was added. After mixing, 0.1 pL of the sample solution was injected directly into a GC or GC/MS (Figure 9) with syringe (Anonymous. 1992; Collins et al., 1997).
gas chromatograph	mass spectrometer
injector
He inlet
detector
column
evacuated chamber
heated oven