Enhancement of lipid production

Due to the various conditions that microorganisms grow and the constant flux of nu­trients that can persist in nature, there are numerous types of lipids found that can change in concentration as the local environments evolve through typical ebbs and flows of materials. In response to these changes, microorganisms will change their cellular struc­tures (i. e., lipid accumulation) by storing energy in various forms in order to utilize exist­ing nutrients and energy to prepare for leaner conditions that may occur. In practical terms, this concept can be leveraged in order to produce high concentrations of intracellu­lar lipids in marine and aquatic biomass in order to maximize the amount of lipids that can be harvested. Several studies have been conducted to determine what conditions af­fect the lipid composition and concentration of microorganisms. The more common tech­niques applied to increase the production of lipids from algae have through genetic manipulation [16], where genetic markers are manipulated that allow for increased lipid production to occur in the cell under normal conditions, by alteration of the cultivation conditions[17, 18], or by addition and manipulation of nutrients and chemicals added to the media [19]. By utilizing methods such as these, algal lipids can be increased by a sub­stantial amount without increasing the footprint of required reactor space, nor greatly in­creasing the amount of time between harvests.