Enzymatic saccharification was done on the pretreated solid wood following the LAP method 009 [16]. The hydrolysis was conducted in 250 mL Erlenmeyer flasks in an oil bath for 3 days in citrate-Na2PO4 buffer (pH 4.8, 100 mL), at 50 °C, and with magnetic stirring (200 rpm). The pH was adjusted using 4M NaOH. The enzyme loading for experimental design samples was 1 mL cellulase (612 u/(g mL), Fisher Scientific, IL, USA). Samples were taken every 24 h to determine sugar content by high performance liquid chromatography (HPLC).

Two commercial enzyme solutions (Cellic CTec2 (1.238 g/mL) and HTec2 (1.209 g/mL), Novozymes North America Inc., NC, USA) were also evaluated as received. Enzyme loading was based on solution weight % (100 x g enzyme solu — tion/g wood)). The enzyme loadings used here were 1.5 % (CTec2 0.06 mL, HTec2

0. 06 mL), 3 % (CTec2 0.12 mL, HTec2 0.12 mL), 6 % (CTec2 0.24 mL, HTec2

0. 25 mL), and 30 % (CTec2 1.21 mL, HTec2 1.24 mL).