Fractionation of Solvated Wood by Non-solvent Addition

Preparation of fractions 1-4 was carried out as follows; Crude fraction 1 was precipitated from IL using acetonitrile as non-solvent. The crude fraction was separated using centrifuge and washed with water, so that filtrate was retained. The solid residue was dried to give fraction 1. For preparation of fraction 2 addi­tional acetonitrile was added into IL-solution followed by similar separation and washing procedure. Fraction 3 was precipitated from IL-solution by water addition, after the acetonitrile had been concentrated by evaporation. Fraction 4 was prepared from the aqueous filtrates retained from the purification of fractions 1 and 2. Fil­trates were concentrated down to a volume ca. 2 mL, which was followed by addition of MeOH. Formed fluffy precipitate was filtered and purified with MeOH. Fraction 5 was prepared as follows. The aqueous IL-solution left from the precipitation of fraction 3 was concentrated so that nearly all water had removed. The remaining IL-solution was precipitated with MeOH (1:10 v/v). Formed precipitation was filtered in grade-3 sinter, washed with MeOH and the filtrand was dried in a vacuum over for 18 h at 40 °C to give fraction 5.