FUNCTIONALIZED RESIN SYNTHESIS AND COMPARISON WITH AMBERLITE

Functionalized weak anion exchange resins used in this study were syn­thesized in the manner stated above, using 6 g of EGDMA, 3 g of IM, and 1 g of DEG (EGDMA:IM:DEG), or 6 g of DVB and 4 g of DMA (DVB:DMA), with a solution of 5 mL of toluene (porogen) and 5 mL 3% acetic acid (dispersing agent). Steady-state binding capacity was deter­mined by agitating 2 g of resin in a 500 mL flask containing 100 mL of KAS603 at 0.4 g/L concentration for 15 min. The suspension containing unbound algae was filtered off and resin transferred into columns. FAME was directly transesterified from the resin as stated previously using 100 mL of 5% sulfuric acid-methanol. For comparison, algae was bound and eluted from a parallel set of Amberlite resin. Because of the higher binding capacity of resin, columns were eluted for 3 consecutive cycles using the same transesterification reagent to ensure complete removal. After reac­tion for 12 h as room temperature, FAME was recovered by extracting twice with 20 mL of hexane. The recovered organic phase was dried by ro­tary evaporation, and the residue resuspended in 1 mL hexane:isopropanol (3:1, v/v) for HPLC analysis.