ALGAL CULTIVATION AND HARVEST

Neochloris oleoabundans (UTEX LB 1185) was obtained from the Uni­versity of Texas at Austin Algal Culture Collection and KAS 603, a saltwater species of Chlorella, was provided by Kuehnle Agro Systems. Neochloris was cultivated in freshwater Bold 3N (B3N) medium [30] us­ing an airlift bioreactor illuminated with cool white fluorescent lights on a 12:12 light:dark cycle and aerated with ambient air using an oil-free diaphragm pump. KAS 603 was cultivated in the same way using f/2 saltwater medium [31]. For nitrogen starvation experiments, Neochloris was grown for 14 days in B3N media, harvested by centrifugation, resuspended in sodium nitrate-free B3N, and cultured for an additional 21 days. For resin binding and FAME synthesis, algae were harvested by centrifugation and resuspended in distilled water to concentration of 0.4 g/L.