The advent of quantum theories on the molecular scale has inspired the de­velopment of electromagnetic exposure systems that mimic the complex interactions and symmetry found in nature from endogenous electromag­netic signals and their destructive interference between interdependent cells. The idea of using multiple interdependent electromagnetic emitters has led into a novel investigation of complex configurations using specific geometric orientations of multiple electrodes generating electromagnetic fields with precise phase orientation and relationships, which may lead to even more significant coupling with biological systems.

The interdependent Multipolar (MP) electromagnetic systems were devised and developed by Lensky [63], and Zavalin and his co-workers [13,14]. The MP system may contain a variety of number of poles, i. e., 2, 3, 5, 6, 9, 12, in the symmetrical electrode configuration (Cn, where n = 2, 3, 5, 6, 9, 12 correspondingly, in notation of the crystallographic groups of symmetry) and complex driving system of interdependent multidimen­sional transformers that is of most importance. For research with bios­timulation of microorganisms, preliminary studies by Zavalin have found that six-pole systems are most effective for microorganisms compared to other configurations. The MP system used in their research consisted of six electrodes in a symmetric hexagonal geometric arrangement (group of symmetry C6), driven by a hexapole interdependent transformer system, powered by an amplified function generator. The frequencies of the EMF oscillations are lower than 100 kHz, providing the near-field regime of the MP EMF during the treatment. The MP EMF generated is fine tuned such that the superpositional field, composed of oscillating electric fields from each electrode in the near-field regime undergoes complete destruc­tive interference with a resultant zero-vector electric and magnetic field within a certain area, located near the center of symmetry and called the “compensation zone”. The compensation zone can produce a “breathing” mode where all coils are energized simultaneously to achieve the multi­polar compensation zone. A scheme for the 6-polar EMF treatment for the test tube culture studies is shown in Figure 2. The multiple pole EMF configurations have a substantial effect on growth of microorganisms. Maximum achieved growth or gas production increases up to approxi­mately 200% (see Figure 3) were observed in various bacteria, yeast, and protozoa under a 5 or 6-pole configuration at 1 kHz [13], 60 Hz, 0.35-2.1 kHz [14]. The AC voltages at the electrodes were applied 180 degrees out of phase for each opposing set of electrodes, resulting in rather pulsating than a rotating EMF pattern. Figure 3 shows maximum increase in growth of E. coli cultures in test tubes under treatment at different frequencies of 6-polar AC EMF. In the plot a maximal achieved ratio of concentration of stimulated E. coli culture to concentration of control E. coli culture at the same conditions is shown in the right vertical axis. A corresponding time, required to achieve such a maximal relative stimulated increase is shown in the left vertical axis. It should be noted that a depression in growth was observed in 2 and 4-pole system at the similar parameters of the EMF at each electrode. The stimulatory effect was greatest in the lag and log phases of the growth curve.

These studies show great promise considering the uniform frequency being emitted was chosen arbitrarily and are open for future research on

the optimization of output signal for growth stimulation. The results of studies conducted by Lensky and Zavalin indicate that higher topologi­cal EMF, having specific group of rotational symmetry is biologically ac­tive. This phenomenon has been previously observed using other types of self-cancelling coil windings [64-66] although the groups of symmetry have not been disclosed. Preliminary evidence indicates that these non­classical designs may be more effective at delivering vibrational informa­tion by coupling with interdependent harmonic oscillating cells because these methods produce relatively large biological effects experimentally [13,14,66]. Thus, the multipolar configuration is a strong prospect for ex­hibiting unique and distinct biological effects.