BIOFUEL PRODUCTION POTENTIAL FROM MICROALGAE

Microalgae are single-cell microscopic organisms which are naturally found in fresh water and marine environment. Their position is at the bot­tom of food chains. Microalgae are considered to be one of the oldest living organisms in our planet. There are more than 300,000 species of micro algae, diversity of which is much greater than plants [3]. They are thallophytes — plants lacking roots, stems, and leaves that have chlorophyll as their primary photosynthetic pigment and lack a sterile covering of cells around the reproductive cells [4]. While the mechanism of photosynthesis in these microorganisms is similar to that of higher plants, microalgae are generally more efficient converters of solar energy thanks to their simple cellular structure. In addition, because the cells grow in aqueous suspen­sion, they have more efficient access to water, CO2, and other nutrients [2, 5]. Generally, microalgae are classified in accordance with their co­lours. The current systems of classification of microalgae are based on a) kinds of pigments, b) chemical nature of storage products, and c) cell wall constituents [2]. Some additional criteria are also taken into consid­eration including cytological and morphological characters: occurrence of flagellate cells, structure of the flagella, scheme and path of nuclear and cell division, presence of an envelope of endoplasmic reticulum around the chloroplast, and possible connection between the endoplasmic reticu­lum and the nuclear membrane [6]. Some major groups of microalgae are shown Table 1.

The oil contents of various microalgae in relation to their dry weight are shown in Table 2. It is clear that several species of microalgae can have oil contents up to 80% of their dry body weight. As mentioned ear­lier, some microalgae can double their biomasses within 24 hours and the shortest doubling time during their growth is around 3.5 hours which makes microalgae an ideal renewable source for biofuel production [7]. The oil content and types of microalgae available at fresh water and ma­rine water are shown separately in Tables 3 & 4.

TABLE 1: Major microalgae groups based on their colours

Colour

Group

1

Yellow-green algae

Xanthophyceae

2

Red algae

Rhodophyceae

3

Golden algae

Chrysophyceae

4

Green algae

Chlorophyceae

5

Brown algae

Phaeophyceae

6

Cyanobacteria

Cyanophyceae

TABLE 2: Oil contents of microalgae [7]

Name of microalgae

(% dry weight)

1

Botryococcus braunii

25-75

2

Chlorella sp.

28-32

3

Crypthecodinium cohnii

20

4

Cylindrotheca sp.

16-37

5

Dunaliella primolecta

23

6

Isochrysis sp.

25-33

7

Monallanthus salina

20

8

Nannochloris sp.

20-35

9

Nannochloropsis sp.

31-68

10

Neochloris oleoabundans

35-54

11

Nitzschia sp.

45-47

12

Phaeodactylum tricornutum

20-30

13

Schizochytrium sp.

50-77

14

Tetraselmis sueica

15-23

TABLE 3: Oil contents of microalgae grown in fresh water [adapted from 2, 7-9, 14-16]

Where Grown

Name of microalgae species

(% dry weight)

1

Botryococcus sp.

25-75

2

Chaetoceros muelleri

34

3

Chaetoceros calcitrans

15-40

4

Chlorella emersonii

25-63

5

Chlorella protothecoides

15-58

6

Chlorella sorokiniana

19-22

7

Chlorella vulgaris

5-58

Fresh Water Algae

8

Chlorella sp.

10 -48

9

Chlorella pyrenoidosa

2

10

Chlorella sp.

18-57

11

Chlorococcum sp.

20

12

Ellipsoidion sp.

28

13

Haematococcus pluvialis

25

14

Scenedesmus obliquus

11 -55

15

Scenedesmus quadricauda

2-19

16

Scenedesmus sp.

20-21

TABLE 4: Oil contents of microalgae grown in marine (salt) water [adapted from 2, 7-9, 14-16]

Where grown

Name of microalgae species

(% dry wt)

1

Dunaliella salina

6 -25

2

Dunaliella primolecta

23

3

Dunaliella tertiolecta

18-71

4

Dunaliella sp.

18 — 67

5

Isochrysis galbana

7- 40

6

Isochrysis sp.

7- 33

Marine Water Algae

7

Nannochloris sp.

20 -56

8

Nannochloropsis oculata

23-30

9

Nannochloropsis sp.

12-53

10

Neochloris oleoabundans

29-65

11

Pavlova salina

31

12

Pavlova lutheri

36

13

Phaeodactylum tricornutum

18-57

14

Spirulina platensis

4 17