In order to achieve large-scale biodiesel production from microalgae, a cost effective cultivation system is of great significance. The cultivation systems include open and closed styles. The former, which simulates the growth environment in natural lakes, is just open-ponds characterized by simple and low cost structure and operations, low biomass concentration, and poor system stability. The closed culture systems are photobioreac­tors (PBR) of different configurations including tubular, flat plate, and column photobioreactors. Compared with open pond systems, the closed systems are usually more stable because it is easier to control the process conditions and maintain monoculture and allow higher cell density, but they have higher capital and operational costs. In both open and closed microalgae culture systems, light source and light intensity are critical to the performance of phototrophic growth of microalgae. With the develop­ment of optical trapping system, light delivery and lighting technologies, which improve the distribution and absorption of light, the advent of some new photobioreactors will improve the efficiency of photosynthesis [17]. In addition, gas-liquid mass transfer efficiency is another critical factor affecting CO2 utilization and hence the phototrophic growth. Cheng et al. [18] constructed a 10 L photobioreactor integrated with a hollow fiber membrane module which increased the gas bubbles retention time from 2 s to more than 20 s, increasing the CO2 fixation rate of Chlorella vulgaris from 80 to 260 mgL-1h-1.