Contaminated of the wide territories in Ukraine not only with radionucludes 137Cs and 90Sr, and with fission products of uranium and transuranium elements is an essential consequence of the accident at the IV block of Chernobyl Nuclear Power Plant that is classified as a global ecological catastrophe. The biota behaviors and adapt in this areas captured dose from radionuclide with long half-value period decay isotopes. As dose related amount of the isotopes 137Cs and 90Sr during long time after accident were decreased. But only the amount of the radioactive isotope 241Am depend of time is increasing exactly in environmental alienation zone of Chernobyl. Radionuclide 241Am as а-emitter is a daughter product of 241Pu isotope appeared after |3-decay. The activity in environment of the isotope 241Am is increasing with during time owing to |3-decay of the 241Pu isotope. The biota behavior in this areas captured dose from radionuclide with long half-value period decay isotopes. The peculiarity of radionuclides contamination associated with the Chernobyl accident is verified of physical and chemical forms of radioactivity elements through out into the environment [Rashydov N. M. 1999, Rashydov N. M., Konoplyova A. A., Grodzinsky D. M. 2004, Rashydov N. M., Kutsokon N. K. 2005, Rashydov N. M., Grodzinsky D., Berezhna V. 2006]. A part of the radioactivity isotopes is registered in water soluble droplets-liquid state, an other part — as "hot" particles, the interrelation between there forms being unstable and change under the influence of biotic and abiotic environmental factors. As rule in this conditions accumulation of radionuclides in plants which occurs mainly at the expense of their water-soluble and exchangeable forms, reflects rather complicated transitional processes in the soil, the rate and direction of these ones is determined by biological activity of all component of the plant rhizosphere inhabited layer of the soil. After Chernobyl accident already during 25 year a lot of "hot" particles transferred into fine dispersive conditions, which easy movements in outdoors where captured by biota which could characterize by help of transfer coefficient (TC) radionuclide ongoing. The transfer coefficient is ratio specific activities (kBq/kg) of plant to specific activity of soil (kBq/kg) where its grow that characterize go over a radionuclide from soil to vegetative plant on experimental plot. Necessary mentioned that the TC not constant and it differed on depend of parts of plant were determinate. For radionuclide 241 Am observed the value TC a lot of plants and mushrooms several order less than for isotopes 137Cs, 90Sr. Especially for matured seed the value of the TC observed less than for other vegetative parts of the plant [Rashydov N., Berezhna V., Kutsokon N. 2007, Rashydov N. M., Kutsokon N. K. 2008, Rashydov N. M., Berezhna V. V., Grodzinsky D. M. 2009, Rashydov N., Berezhna V. 2010, Rashydov N. M. 2010, Rashydov N. M. 2011]. To study of the TC peculiarity modification is reason elucidation of our field research in alienation Chernobyl zone around object "Shelter".

Contamination of plant in natural experimental fields at the alienation zone of Chernobyl significant added by flying dust with very small size radioactivity particles less than "hot particles" in environment. The results received for plants soybean (content: 137Cs — 3.6 kBq/kg and 90Sr — 11.84 kBq/kg) and flax (content: 137Cs — 0.78 kBq/kg and 90Sr — 3.55 kBq/kg) which grown in Chistogalovka (specific activity of soil is 20.65 kBq/kg and 5.18 kBq/kg for radionuclide 137Cs and 90Sr, accordingly) and Chernobyl confirmed this hypothesis. The value TC for above mentioned seeds specimens collected from plant which grow on Chistogalovka was approximately 22.3 (soybean) and 6.63 (flax) times (for isotope 137Cs) and 13.97 and 4.71 (for isotope 90Sr) times higher by comparison with control variants which grown in Chernobyl where specific activity was 1.41 kBq/kg for radionuclide 137Cs and 0.55 kBq/kg for isotope 90Sr, correspondingly.

The peculiarity distribution in controlled laboratory conditions the radionuclide 241 Am in Arabidopsis thaliana plant on high level first layer leaves, in petiole and in carry out fascicles of the leaves significantly that go into this isotope from root system to top of plant very slow and membrane of cells played as discrimination barrier in this processes as mentioned in our previously investigations [Rashydov N. M., Berezhna V. V., Grodzinsky D. M. 2009].

In laboratory conditions for autoradiography investigation purpose the seedlings Arabidopsis thaliana were aseptically grown in hard agar cultured medium containing 241AmCl3 in concentration with specific activity 50 kBq/kg. After 25 days some leaves and top of stems of plants witch had not direct contact with medium were carefully cut off so that to avoid contact with medium. Selected parts of plants Arabidopsis thaliana settled down on the microscopic glass slides and dried a few days. During this process they were gluing to the slides themselves. The slides with parts of plants were coated with photo emulsion LM-1 in gel (Amersham — Biosciences UK) and exposure during time 20 days at temperature +40 C. After development the samples of slides were observed of the track of a-particles from radionuclide 241 Am with light microscope. A lot of datum confirmed that the coefficient
uptake very small for radionuclide 241Am and this element maldistribution by organs and tissues. We observed that accumulation the radionuclide of 241Am depended of carry out fascicles system of the leaves and localization of the layer leaves not far from length root collar of plant which grow in laboratory conditions. The first layer leaves were taken up high-level amount radionuclide 241 Am. As result the capture dose also may tissues of plant distribute no uniform. It is known that mineral nutrients are transported apoplastically, i. e. in the wall system outside the plasma membrane, or symplastically, i. e. in the cytoplasm from cell to cell deal with through plasmodesmata. The nutrient elements that penetrate into the cytoplast can also be shuttled into the vacuole via various mechanisms depending of biological function in cell life behaviors for mentioned isotope.

For field experiments we use plant white blow (Erophila verna (L.) Bess.) for autoradiography investigation from Chistogalovka and Yaniv contaminated soil sites the distribution radionuclide essential differs in spite of above-mentioned experiment. On the top shoot apex leaves and flower observed a lot of tracks of the particles a — and P — decays [Rashydov N. M., Berezhna V. V. 2010] (Figure 36).

Our experimental data confirms that radioactivity fallout in environment essentially differed important amendment of the TC. Thus extra-root nutrition that included micro — or nano- size "hot" particles had essential role of plant behavior in environment. But for plants that harvested from contaminated sites distribution of the radionuclide 241Am by tissue and organs essentially differed from plants which grown in laboratory conditions.

(a) (b)

Contamination with radionuclide in natural experimental fields significant added tracks elementary particles from flying in air very small dust such as nano — and micro-size with radioactivity similarly "hot" particles in environment by help foliar pathway uptake into top leaves and aboveground apical apex of plants, especially around the object "Shelter".