Microorganisms

One of the main industrial uses of microorganisms has been alcoholic fermenta­tion. The giant “microbial libraries” in current vogue can be studied for microbes that convert cheaper carbohydrates into value-added products, which can serve as raw materials for the fermentation of hemicellulosic-derived sugars into valuable commercial commodities [30]. The bioconversion process holds more promise of utilizing both hexose and pentose sugars from lignocellulosic materials. Microbial

Microorganism

Enzyme

Substrate

Specific activity (p mol min-1 mg-1)

Mechanistic applications

Bacteria

Fibmbacter succinogenes

Acetyl xylan esterase

Acetylxylan/alpha-naphthyl

acetate

2,933

Hydrolyze the acetyl substitutions on xylose moieties

Thennoanaerobacter

ethanolicus

Beta-1,4-xylosidase

o-nitrophenyl-beta-D-

xylopyranoside

1,073

Hydrolyse xylobiose; release xylose

Bacillus polymyxa

Beta-Glucosidase

4-nitrophenyl-beta-D-

glucopyranoside

2,417

Act upon Beta-Glucosidase to release glucose

Bacillus subtilis

Endo-alpha-1,5-arabinanase

1,5-alpha-L-arabinan

429

hydrolase activity, hydrolyzing O-glycosyl compounds

Escherichia coli

alpha-Galactosidase

Raffinose

27,350

Hydrolyzes the terminal alpha-galactosyl moieties from xylans

Clostridium stercorarium

Feruloyl esterase

Ethyl ferulate

88

Hydrolyze the ester bond between the arabinose substitutions and ferulic acid

Bacillus subtilis

Endo-galactanase

Arabinogalactan

1,790

Release of L-arabinose substituted

D-galactooligosaccharides from arabinogalactan

Bacillus subtilis

Endo-beta-1,4-mannanase

Galactoglucomannan/

glucomannans/mannan

514

Acts upon interior side of beta-1,4-mannan to yield mannose

Fungi

Phanerochaete chiysosporiwn

Alpha-Glucuronidase

4-O-methyl-glucuronosyl-

xylotriose

4.5

Hydrolyses Alpha-1,2 Glycosidic bond the 4-O-methyl-D-glucuronic acid sidechain of xylans

Table 2 Hemicellulase titers from different microorganisms and their mechanistic applications (Source: Howard et al. [29].)

Biotechnological Applications of Hemicellulosic Derived Sugars

conversion of hexose sugars into chemicals is well established; however, the ability of these organisms to ferment pentose sugars is somewhat less so. The use­ful exploitation of lignocellulosics by fermentation can be enhanced by efficient utilization of the pentosanic fraction along with hexoses.

Yeasts that have been studied extensively for use in xylose fermentation include Pachysolen tannophilus, Candida shehatae, Pichia stiptis, and Kluveromyces marxi — anus [3]. The optimal performance of these microorganisms is usually controlled by the air supply. Other yeasts investigated for their xylose-fermenting ability include Brettanomyces, Clavispora, Schizosaccharomyces, several other species of Candida viz. C. tenius, C. tropicalis, C. utilis, C. blankii, C. friedrichii, C. solani, and C. parapsilosis, and species of Debaromyces viz. D. nepalensis and D. polymorpha. Maleszka and Schneider [31] screened 15 yeast strains for their ability to utilize D-xylose, D-xylulose, and xylitol for ethanol production under aerobic, microaero­bic (low aeration), and anaerobic conditions using rich undefined or defined media. In almost all cases, ethanol production by P. tannophilus and species belonging to Candida and Pichia was better on rich media under microaerobic conditions [3,4, 31].

Several pentose-utilizing fungal species like Fusarium oxysporum, Rhizopus sp., Monilia sp., Neurospora crassa, Paecilomyces sp., Mucor sp., Neurospora crassa, and F oxysporum and bacterial species like Bacillus macerans, B. polymyxa, Kiebsiella pneumoniae, Clostridium acetobutylicum, Aeromonas hydrophila, Aerobacter sp., Erwinia sp., Leuconostoc sp., Lactobacillus sp., Clostridium ther — mocellum, C. thermohydrsulfurium, C. thermosaccharolyticum, and C. thermosul — furogenes utilizing pentose, hexose, and lignocellulose hydrolysates for ethanol production have been extensively reviewed [32].