Plant biomass is an important factor in the study of functional plant biology and growth analysis, and it is the basis for the calculation of net primary production and growth rate. The conventional means of determining shoot dry weight (SDW) is the measurement of oven-dried samples. In this method, tissue is harvested and dried, and then shoot dry weight is measured at the end of the experiment. For the measurement of biomass of a large number of plants, this method is time consuming and labor intensive. Also, since this method is destructive, it is impossible to take several measurements on the same plant at different time points. With the establishment of advanced technology facilities for high throughput plant phenotyping, the problem of estimating plant biomass of individual plants is becoming increasingly important. There are several technologies that can help to assess the effect of abiotic stresses like drought and soil salinity on plant growth while assisting in predicting crop yield under various environmental conditions.