Green Extraction Techniques for Bioactive Compounds

Today, there is a wide range of classical or conventional extraction techniques that have been traditionally employed for the extraction of interesting compounds from natural matrices, such as algae. In this group, techniques such as Soxhlet, liquid-liquid extraction (LLE), solid-liquid extraction (SLE), and other techniques based on the use of organic solvents are included. Although these techniques are routinely used, they have several well-known drawbacks; they are time consuming, laborious, they lack of automation and therefore are more prone to present low reproducibility, have low selectivity and/or provide low extraction yields. These shortcomings can be partially or completely overcome by using the newly developed advanced extraction techniques. This new kind of extraction techniques are characterized by being faster, more selective towards the compounds to be extracted, and also very important nowadays, these techniques are more environmentally friendly. In fact, by using the considered advanced extraction techniques, the use of toxic solvents is highly lim­ited. In the next sections, the most important advanced extraction techniques that have been employed to extract bioactive compounds from algae are briefly described and commented.