Actually, there are known many different techniques used for biomass extraction: liquid — solid extraction, liquid-liquid extraction, partitioning, acid-base extractions, ultrasound extraction (UE), microwave assisted extraction (MAE). The capability of a number of extraction techniques have been investigated, such as solvent extraction (J. A. Saunders, D. E. Blume, 1981) and enzyme-assisted extraction (B. B. Li, B. Smith, M. M. Hossain, 2006). However, these extraction methods have drawbacks to some degree.

The choice of extraction procedure depends on the nature of the natural material and the components to be isolated. The main conventional extraction procedures are liquid-liquid extraction and liquid-solid extraction. For liquid-liquid extraction is using two different solvents, one of which is always water, (water-dichloromethane, water-hexane, and so). Some of the disadvantages of this method are: cost, toxicity and flammability (Kaufmann 2002; McCabe, 1956; Perry, 1988; Sarker et. al., 2006).

Solid-phase extraction (SPE) can be used to isolate analytes dissolved or suspended in a liquid mixture are separated from a wide variety of matrices according to their physical and chemical properties. Conventional methods include: soxhlet extraction, maceration, percolation, extraction under reflux and steam distillation, turbo-extraction (high speed mixing) and sonication. Although these techniques are widely used, have several shortcomings: are very often time-consuming and require relatively large quantities of polluting solvents, the influence of temperature which can lead to the degradation of thermo labile metabolites (Kaufmann 2002; McCabe, 1956; Sarker et. al., 2006; Routray, 2012).

Supercritical fluid extraction (SFE), microwave-assisted extraction (MAE) and pressurised solvent extraction (PSE) are fast and efficient unconventional extraction methods developed for extracting analytes from solid matrixes.