Sources of further information

Basic information on the kinetics of enzymatic reactions is available in the books of Bailey and Ollis (1986), Dutta (2008) and Shuler and Kargi (2001). These books present a biological background and provide a comprehensive introduction to biochemical engineering. Introduction to the genetic sequencing for producing proteins from recombinant DNA is also available in these books. However, an interested reader should refer to more specialized books (Martin and Christopher, 1990; Leskovac, 2003; Cook and Cleland, 2007) for more profound information, where much more is included about the structures of enzymes and the kinetics and mechanisms of enzymatic reactions. Enzymatic kinetics mechanism, relative rates of steps along the reaction pathway, and chemical mechanism, including acid-base chemistry and transition state structure for mono-, bis — and tri-substrate reactions are explained, and numerous general experimental protocols and kinetic data interpretation are described. In addition, a comprehensive catalog of enzymes in general, and lipase in particular, and their uses in modern manufacturing are available in the book of Polaina and Maccabe (2007) and the book of Uhlig (1998). These books survey general enzyme characteristics and discuss their microbiological origin, and stability of each enzyme. In addition, the most important industrial enzymes in use today are examined including immobilized enzymes.

As far as biodiesel is concerned, the book of Pahl (2005), ‘Biodiesel: Growing a New Energy Economy’, offers a comprehensive review from the history of the diesel engine to the development of the biodiesel industry, past, current and future. In addition, detailed information and news updates are available on the webpage of

the National Biodiesel Board (NBB) (http://www. biodiesel. org/). NBB is the

national trade association representing the biodiesel industry in the United States.