Diversity of plant cell wall structures

The plant cell wall structures are highly diverse. Various lignocellulosic species have been used for biofuels production, woods, crop by-products, herbaceous plants, beet pulp, municipal and paper industry wastes. Although all these different biomasses contain typically four major components (i. e. cellulose, hemicelluloses, pectin and lignin), the architecture of the cell wall, the fine biochemical structures of these components and their interactions into the cell wall could be quite different. Nevertheless, cellulose and hemicelluloses leading, with lignins, to the formation of an insoluble, tridimensional network is a constant behavior. A schematic drawing of the plant cell wall polysaccharides is shown in Fig. 1.

image140

Fig. 1. Schematic representation of plant cell wall polysaccharides. Cellulose and P-1,3/1,4- glucan are composed of glucose residues (red). P-1,4-xyloglucan is a glucose-based polymer substituted by xylose residues (green) themselves possibly linked to galactose (blue) and fucose (black). P-1,4-arabinoxylan is a xylose backbone linked with arabinose (orange), and/or with glucuronic acid acid (white). Xylose could be substituted by acetyl groups (blue circle). P-1,4-mannan is a mannose polymer (dark red) linked to some galactose residues and sometimes acetylated. a-1,4-polygalacturonan is formed of linear chains of galacturonic acid (pale yellow) linked by rhamnose (pink). Galacturonic residues could be either methylated (red circle) or linked to xylose residues. Rhamnogalacturonan is highly ramified and is also called "hairy region" for this reason. The basic backbone is a rhamnose-galacturonic acid motif. Side chain of arabinose, galactose, mixed or not, linear or not, forms a very complex and variable structure. Adapted from Dalboge (1997).

As another example of variable composition of plant cell wall, lignins and sugars in cell walls of different origins were quantified (Table 1). Although this study is not exhaustive, it is clear that every plant has its own characteristic. These biomasses have been used as growth substrates for Fusarium graminearum (see paragraph 4).

Regarding cell wall composition variation, it could be postulated that cell wall degradation recalcitrance could be related to cell wall structure and ultra structure variability.

Hop

Wheat bran

Corn cops

Birch

Lignin

30.9

17.3

6.5

18.1

Total neutral sugars

35.2

50.1

63.5

57.6

Glucose (i. e. glucans)

20.1

14.8

28.8

40.0

Xylose (i. e. xylans)

2.7

15.0

21.0

15.6

Table 1. Lignin, glucose and xylose contents of hop, destarched wheat bran, corn cops and birch. Results are expressed as % of dry matter (unpublished results from the laboratory). Klason lignin was quantified as the acid-soluble residue after sulfuric acid hydrolysis (Remond et al. 2010) and sugar contents were estimated by enzymatic methods as described in Phalip et al. (2009).