A lava rock biofilter was used to evaluate the degradation of H2S from the AD gas stream. The experimental setup for the biofilter used in this study was previously described (Ramirez-Saenz et al 2009). The gas stream was humidified and fed in the top of the biofilter using a mass flow controller. Sample ports were located in the output and input of the gas stream. For H2S degradation experiments, the biofiltration system was fed at the top with an air-diluted gas stream originated from the ADR, as previously reported (Ramirez-Saenz et al., 2009). Periodic water additions (once a week) were used to control moisture loss and to avoid SO4-2 accumulation. Recirculation was provided at a flux of 0.5 L/min over 1 h. All experiments were conducted at room temperature (20-25°C).