Vitality of the yeast cells

Fig. 8 shows the yeast cells after a glucose fermentation. A large part of the yeast cells have become inactive, even though there is still enough sugar in the substrate to sustain living cells.

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Fig. 8. Methylene blue stained yeast cells after glucose fermentation.

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Fig. 9. Methylene blue stained yeast cells after heating to 100 °С.

During the experiments with dose response on furfuryl alcohol in glucose fermentation, a relation was found between cell death and the concentration of furfuryl alcohol (Fig. 8). Yet at this writing, there still exist some inconsistencies between erioglaucine and methylene blue. Overall the results produced by methylene blue appeared slightly more consistent and matching with the data for ethanol production.