Construction of network model

The metabolic network encompasses all the primary reaction routes involved in the anaerobic growth of recombinant yeast such as glycolytic and pentose phosphate pathways, citric acid cycle, and reactions for pyruvate metabolism. In addition, two oxidoreductase reactions from xylose to xylulose catalyzed by the heterologous expression of XR and XDH enzymes are incorporated. Biochemical reactions participating in the metabolism of recombinant yeast are listed up in Table 2.1.