Cassava bioethanol wastes and their utilization

During cassava bioethanol production, wastes are generated; the quantity and quality are depending significantly on feedstock quality and processing types. Since dry milling process is more widely used for bioethanol production from cassava feedstock, the information provided here is based on dry milling process of cassava chips. Similar to dry milling process for bioethanol production of corn grains, both of solid and liquid wastes are obtained at the end of distillation. The waste can be generated as a whole stillage containing both solid and liquid waste if the whole beer is subjected to the mash column without fiber separation. This process is applied in order to minimize ethanol loss in the solid pulp if fiber separation is accomplished prior to distillation. Recently, the process is adjusted by separating the fiber first and the fiber is washed to collect ethanol in pulp. At the production capacity of 150,000 liters of anhydrous ethanol/day, the total whole stillage is produced approximately 1,400-1,600 m3/day, being wet cake 100-200 ton/day and the stillages 1,200­1,400 m3/day (Sriroth et al., 2006).

Parameters

Values

SSF1

SLSF2

Slurry

Volume (L)

2,053

2,200

% Total solid (w/v)

24.18

24.24

% Starch content of chips

80.4%

74.49%

ph

4.68

4.45

Beer after fermentation

Fermentation time (hrs)

48

60

Volume (L)

2,166

2,258

Total soluble solid (oBrix)

12.2

7.4

Glucose content (%w/ v)

1.09

1.24

Ethanol content (%w/v)

8.66

8.18

Cell counts (x 107 cell/ml)

6.82

1.15

Yield

g ethanol/g dried chips

0.378

0.344

g ethanol/g starch

0.470

0.462

%Fermentation efficiency3

82.88

82.11

1 Using 25% dry solid of chips, liquefied by 0.1% Termamyl 120L (Novozymes) at 95-100°C, 2 hr followed by simultaneous saccharification and fermentation with 0.1% Rhizozyme (Alltech) or AMG (Novozymes) and Saccharomyces cirivisiae at 32°C for 48 hrs.

2 Using 25% dry solid of chips, liquefied, saccharified and fermented with 0.25% granular starch hydrolyzing enzyme (Stargen™, Danisco-Genencor, USA) and Saccharomyces cirivisiae at 32°C, 60 hr.

3 as a percentage of theoretical yield

Source: Rojanaridpiched et al., 2003 ; Sriroth et al., 2007

Table 8. Parameters and results of ethanol production from cassava chips by SSF and SLSF process.