For endophytic fungal growth, surface-sterilized tissues are cut into small pieces or homogenized using a homogenizer and plated on Potato dextrose agar (PDA) or other fungal culture media, such as MEA medium (2% malt extract and 1.8% agar) (Vallino et al. 2009) supplemented with several antibiotics, such as ampicillin (100 mg/L), chloramphenicol (50 mg/L) and streptomycin (50 mg/L) to prevent bacterial growth (Ghimire et al. 2011; Craven K, personal communication). Plates containing tissues are incubated at 25-28°C for up to one month. Observations should be taken every day in order to isolate individual strains for further identification. The growing fungal colonies are then re-plated on fresh medium to obtain individual colonies.