Как выбрать гостиницу для кошек
14 декабря, 2021
я-Butanol has favorable properties as a gasoline blending agent and provides a valuable target to validate R. eutropha as a host for synthetic biology [14]. The PHB synthesis pathway in R. eutropha proceeds through 3-hydroxybutyryl-CoA, which is also an intermediate in the я-butanol synthesis pathway. Our strategy will be to divert the flux from 3-hydroxybutyryl-CoA to PHB and to redirect it to я-butanol. Recent work has demonstrated that high titers of я-butanol can be produced in E. coli by choosing heterologous genes judiciously and maximizing reducing equivalents available for я-butanol production [2]. Therefore, a detailed understanding of the expression of heterologous genes for я-butanol production in R. eutropha and the metabolic flux in R. eutropha PHB — mutants will be essential in achieving high titers of я-butanol.
Although я-butanol can be used directly as a gasoline replacement due to its higher energy content and lower water solubility and corrosivity relative to ethanol, it would only address short-haul ground transportation, since it could not be used to power aircraft or long-range rail and trucks. Dehydration of butanol to butylenes (C4) and oligomerization affords C8, C12 and C16 olefins with some disproportionation to non-oligomer C9 , C10, C11, C13, C14, and C15 olefins. These olefins can undergo double-bond isomerization, skeletal isomerization, cyclization and/or aro — matization, forming isoalkenes, cycloalkenes, and/or aromatic products. Hydrogenation of this mixture may provide hydrocarbons suitable for use as jet fuels. We will transform the я-butanol obtained from H2/CO2 cultivation of engineered R. eutropha to these hydrocarbon mixtures and evaluate them as replacement for jet fuel. We are currently exploring novel catalysts for the dehydration and oligomerization of butanol to hydrocarbon mixtures that resemble jet fuel.