Plants of experimental field plots were harvested at physiological maturity (growth stage 90 DC) by a harvester thresher HEGE 140 (Hans-Ulrich Hege GmbH & Co, Germany). After the harvest, the grains were cleaned on the sieves by flow of air and then yield and thousand — grain weight were determined. Analytical samples were made according to methodology ISO 13690:1999 by quartering of cleaned grain. A common (average) analytical sample of four trial plots grain was prepared.

Sampling from the experimental pots was performed three weeks after the application of brassinosteroids in plant growth stages referred to a decimal code for the growth stages of cereals. The first sampling was performed in the plant growth phase 47-49 DC (visible awns), the second sampling in the growth stage 73-75 DC (30-50 % of final grain size). Grain and straw samples were taken in the growth phase Z90-92 (full ripeness). Green plants were taken from experimental pots cleaned up with distilled water and subsequently freeze-dried.